Melanin expression in Paracoccidioides brasiliensis: chemical induction with L-DOPA and L-epinephrine
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Abstract
Introduction. Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis (PCM), one of the most important mycoses in Latin America, it is a thermo dimorphic fungi that growth at 36°C (yeast-parasitic form) and 18ºC (mycelia- saprophytic form).
Objective. To determine in vitro the expression of melanin by P. brasiliensis using L-DOPA and Lepinephrine chemical factors. Materials and methods. ATCC 60855 P. brasiliensis strain growth in pH 5,5 synthetic media supplemented with L-dihidroxifenilalanina or L-epinephrine.
Results. Was observed that chemical induction with L-DOPA 1mM or L-epinephrine 0,8 mg/ml generated melanization of the P. brasiliensis yeast.
Conclusions. P. brasiliensis yeast could growth in vitro in acid media (pH 5,5) and synthesizes melanin using external chemical induction as L-DOPA or L-epinephrine.
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References
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