Expresión de melanina en Paracoccidioides brasiliensis: inducción química con L-DOPA y L-epinefrina

Martha Eugenia Urán J.; Lina Marcela  Castañeda A.; Ángela Restrepo M.; Luz Elena Cano R.

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Published: 2008-06-15

How to Cite

Urán J., M. E., Castañeda A., L. M., Restrepo M., Ángela, & Cano R., L. E. (2008). Melanin expression in Paracoccidioides brasiliensis: chemical induction with L-DOPA and L-epinephrine. Medicina UPB, 27(1), 17-24. https://revistas.upb.edu.co/index.php/medicina/article/view/2247

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Martha Eugenia Urán J., Corporation for Biological Research

Unit of Medical and Experimental Mycology, Corporation for Biological Research (CIB), Medellín, Colombia.

Lina Marcela Castañeda A., College of Antioquia

University Institution Colegio Mayor de Antioquia.

Ángela Restrepo M., Corporation for Biological Research

Unit of Medical and Experimental Mycology, Corporation for Biological Research (CIB), Medellín, Colombia.

Luz Elena Cano R., University of Antioquia

Faculty of Microbiology, University of Antioquia, Medellín, Colombia.

Martha Eugenia Urán J.

Corporation for Biological Research

Lina Marcela Castañeda A.

College of Antioquia

Ángela Restrepo M.

Corporation for Biological Research

Luz Elena Cano R.

University of Antioquia

Abstract

Introduction. Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis (PCM), one of the most important mycoses in Latin America, it is a thermo dimorphic fungi that growth at 36°C (yeast-parasitic form) and 18ºC (mycelia- saprophytic form).

Objective. To determine in vitro the expression of melanin by P. brasiliensis using L-DOPA and Lepinephrine chemical factors. Materials and methods. ATCC 60855 P. brasiliensis strain growth in pH 5,5 synthetic media supplemented with L-dihidroxifenilalanina or L-epinephrine.

Results. Was observed that chemical induction with L-DOPA 1mM or L-epinephrine 0,8 mg/ml generated melanization of the P. brasiliensis yeast.

Conclusions. P. brasiliensis yeast could growth in vitro in acid media (pH 5,5) and synthesizes melanin using external chemical induction as L-DOPA or L-epinephrine.

Keywords:

Melanins, Paracoccidioides, Levodopa, epinephrine, Paracoccidioidomycosis, Fungi, Virulence factors

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