Operative characteristics of real time polymerase chain reaction and loop mediated isothermal amplification in the detection of pulmonary tuberculosis: A systematic review

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Published: 2011-06-15

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Serna Quintana, G. J., & Aristizábal Bernal, B. H. (2011). Operative characteristics of real time polymerase chain reaction and loop mediated isothermal amplification in the detection of pulmonary tuberculosis: A systematic review. Medicina UPB, 30(1), 36-47. https://revistas.upb.edu.co/index.php/medicina/article/view/1917
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Gabriel Jaime Serna Quintana
Clinical Laboratory Prolab S.A.S.
Beatriz Helena Aristizábal Bernal
Hospital Pablo Tobon Uribe image/svg+xml

Abstract

Objective: to describe the operating characteristics of the real-time Polymerase Chain Reaction (qPCR) and Loop Mediated Isothermal Amplification (LAMP) for the diagnosis of pulmonary tuberculosis caused by the members of Mycobacterium tuberculosis complex.


Methods: systematic review of the scientific literature published during the years 2005 to 2009, of LAMP test compared to qPCR for the diagnosis of pulmonary tuberculosis.


Results: the findings showed sensibilities among 94.1% to 100% for LAMP tests, compared to 85.2% to 86.3% for qPCR test, and specificities between 94.2% - 100 % for LAMP as opposed to 88.6% – 100% for qPCR test.


Conclusions: these findings demonstrate the relevance of evaluating the above mentioned tests in our region to improve the diagnosis of this disease.

Keywords:
Review, Mycobacterium tuberculosis, tuberculosis, pulmonary, nucleic acid amplification techniques, diagnosis

Article Details

Author Biographies

Gabriel Jaime Serna Quintana, Clinical Laboratory Prolab S.A.S.

Bacteiologist and clinical laboratory worker, Laboratorio Clínico Prolab S.A.S. Master's student in Microbiology and Bioanalysis. University of Antioquia. Researcher at the Center for Epidemiology and Molecular Diagnosis of Infectious Diseases (Ediemi). Medellin Colombia.

Beatriz Helena Aristizábal Bernal, Hospital Pablo Tobon Uribe

MSc PhD in molecular biology. Molecular Diagnostic Laboratory, Hospital Pablo Tobón Uribe. Researcher at the Center for Epidemiology and Molecular Diagnosis of Infectious Diseases (Edimei).

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